There are many names for ELISA kits in China, such as ELISA detection kits ELISA Kit、 Enzyme linked immunosorbent assay kit, enzyme-linked immunosorbent assay kit, enzyme-linked immunosorbent assay kit, enzyme immunoassay kit, etc., commonly referred to as ELISA detection kit, enzyme-linked immunosorbent assay kit, etc; The ELISA kit is suitable for in vitro qualitative detection of IgM antibodies against human hepatitis E virus (HEV) in human serum or plasma. Since its introduction in the 1960s and 1970s, it has been recognized and praised by researchers around the world, and has been widely promoted in Europe, America, and China, especially in the field of biochemistry, which has made significant progress in China. Elisa biological assay is a highly sensitive, specific, and reproducible experimental diagnostic method. Due to its stable reagents, easy storage, simple operation, and objective result judgment, it has been widely used in various fields of immunological testing. In order to ensure the quality of the ELISA kit experiment, we need to master some tips to help the success of the experiment. Do you know the operation skills of the ELISA kit?

Today, Genmei will tell you 17 operating techniques for ELISA kits. If you master these experimental techniques, you will be more proficient in conducting ELISA experiments, greatly reducing the risks during the experiment. As a beginner in ELISA kit experiments, mastering these knowledge will make the operation much faster.

The 17 related operation tips for ELISA kit are as follows:

1. Remember to gently wipe and dry the enzyme labeled plate surface with absorbent paper after adding the enzyme reagent.

2. Reasonably arrange the amount of testing to avoid too many reaction plates causing long waiting times for plate washing.

3. When sucking liquid, use a gun with a range close to the required amount to reduce errors.

4. It is necessary to conduct a double well experiment to ensure the accuracy of the data and reflect the precision of the reagent kit.

5. The sample diluent should be added using a dispenser and its accuracy should be regularly checked.

To prevent sample evaporation, place the reaction plate in a sealed box covered with a damp cloth during the experiment, and cover or film the enzyme-linked immunosorbent assay plate.

7. Unused enzyme-linked immunosorbent assay (ELISA) plates or reagents should be stored at 2-8 ℃. Please prepare and use horseradish peroxidase labeled anti human IgG working solution according to the required amount. Do not reuse diluted horseradish peroxidase labeled anti human IgG working solution.

In the ELISA kit experiment, after adding the sample, it should be placed in the incubator in a timely manner. If there are many samples, they should be operated in batches. Strictly control the operation time according to the instructions to prevent artificially prolonged incubation time, which may cause non-specific binding to adhere tightly to the reaction well and make it difficult to clean thoroughly.

9. The remaining samples and waste should be sterilized with high-pressure steam at 121 ℃ for 30 minutes, or treated with disinfectants such as 5.0g/L sodium hypochlorite for 30 minutes before being discarded.

10. When washing the human ELISA kit, each well should be filled with liquid to prevent any free enzymes from washing the well.

11. Leave one hole as a blank zeroing hole for each experiment. No reagents are added to this hole, only the substrate solution and 2N H2SO4 are added at the end. When measuring, first use this hole to adjust the OD value to zero.

12. When manually washing boards, add detergent each time. It should be left to stand for 15-30 seconds, and the washing solution from one enzyme labeled well should not be splashed into another enzyme labeled well to prevent cross contamination. After shaking off the detergent, place the enzyme-linked immunosorbent assay (ELISA) plate on a towel or absorbent paper and pat dry.

When there are doubts about the results of the sample, other testing methods need to be used for verification.

When there is no deionized water or double distilled water, Wahaha purified water can be used to prepare the solution, and tap water should not be used.

15. When using a micro sampler, the nozzle should be replaced after extracting liquids from different bottles, even if standard solutions are being extracted.

16. When sucking liquid, the speed should not be too fast to avoid the generation of bubbles, and the amount sucked by the human ELISA kit may not be accurate enough.

17. When suctioning liquids, it is necessary to use a micro sampler with a range close to the required amount to reduce errors.